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Experimental thermal melting temperatures (TM) for 8-azaN duplexes.
| 8-azaN Duplex | TM (°C)† |
|---|---|
| H16 | 62.5 ± 0.1 |
| GC-rich | 88 ± 1 |
| AU-rich | 25.5 ± 0.1 |
| H14 A | 54.6 ± 0.9 |
| H14 B | 55.1 ± 0.1 |
| XA | 58.0 ± 0.7 |
| XA+ | 81.3 ± 0.1 |
| XB | 20.9 ± 0.5 |
| XB+ | 58.4 ± 0.0 |
TM values were measured at the following conditions: 1 μM duplex, 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 100 mM NaCl, and 1.25 μM EvaGreen. Values reported are the average of three independent measurements ± standard deviation.
