TABLE 2.
Clearance of Chlamydia from the female genital tract in iNOS knockout and immunocompetent control micea
| No. of wk postinfection | iNOS+/+ mice
|
iNOS−/− mice
|
||
|---|---|---|---|---|
| % Infected | No. of IFU (mean)b | % Infected | No. of IFU (mean)b | |
| 1 | 100 (12/12)c | 7.6 × 104 | 100 (12/12) | 1.6 × 105 |
| 2 | 100 (12/12) | 1.5 × 104 | 100 (12/12) | 7.0 × 103 |
| 3 | 91.7 (11/12) | 5.9 × 103 | 41.6 (5/12) | 1.3 × 101 |
| 4 | 8.3 (1/12) | 8.6 × 101 | 0 | 0 |
| 5 | 0 | 0 | 0 | 0 |
a
iNOS+/+ mice were (C57BL/6 × 129/J)F2; iNOS−/− mice were a third-generation random intercross of C57BL/6 mice homozygous for a 129/SvEv embryonic stem-derived mutant iNOS gene. Therefore, both iNOS+/+ and iNOS−/− mice are segregating for all other loci.
b
Mean number of recoverable chlamydial IFU from the indicated number of mice at selected time points following intravaginal infection with MoPn as described in Materials and Methods.
c
Values in parentheses indicate the number of mice infected relative to the total number of mice.