Figure 2.

Clz potentiates adipogenic differentiation, as indicated by intracellular lipid accumulation, in response to a threshold-level stimulus. UD 3T3-L1 cells were exposed to a threshold-level adipogenic stimulus alone (“Positive control” or “0 μM Clz”) or in combination with the various Clz concentrations indicated (as described in the methods section). A. At 14 days PI, photomicrographs were obtained of cultures at 100x (left-hand panels, intracellular lipid as characteristic golden spheres). Cultures were terminated and ORO-stained; after staining, photomicrographs of cultures were again obtained at 100x (right-hand panels). Photomicrographs are from a single biological replicate, but are broadly representative in trend of n = 6 total biological replicates. B. Quantitative data for ORO staining of Clz-treated cultures, obtained at 14 and 17 days PI. Data (mean ± SEM) are expressed as fold change versus internal positive control (0 μM Clz), with internal negative control as background, for each biological replicate (n = 6 for D14, n = 3 for D17); *p<0.05 by Dunnett’s post-test.