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. Author manuscript; available in PMC: 2024 Jan 23.
Published in final edited form as: Sci Immunol. 2023 Aug 25;8(86):eadg0878. doi: 10.1126/sciimmunol.adg0878

Fig. 1: B7 costimulation is required for the maintenance of virus-specific CD8 T cells during chronic LCMV infection.

Fig. 1:

(A) Experimental layout: C57BL/6J mice were transiently depleted of CD4 T cells and infected with LCMV clone 13. Mice with established life-long chronic infection (>40 days post infection) remained untreated (UNT) or received anti-B7–1 and anti-B7–2 blocking antibodies (αB7) during 7 weeks (wks). (B and C) Frequency of LCMV-specific CD8 T cells in spleen. (D and E) Number of LCMV-specific CD8 T cells in different organs. (F and G) Ki-67 expression on LCMV-specific CD8 T cells in the spleen. (H) Viral titer in organs, as quantified by plaque assay. PFU, plaque-forming units. (I) Number of splenic LCMV-specific Tpex and Tex. (J) Frequency of Tpex (TCF-1+) and Tex (TIM-3+) virus–specific CD8 T cells in spleen. Data in (B, C, F, G, H and J) are representative of 3 independent experiments with 4–5 mice/group. Data in (D, E, and I) show combined data from two of three independent experiments. Symbols represent individual mice, bars show mean value of all animals analyzed and error bars indicate SEM. Significance was determined using unpaired Student’s t-test *P < 0.05, **P < 0.01, ***P < 0.001. ns, not significant.