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. 2023 Dec 31;72(6):753–765. doi: 10.33549/physiolres.935129

Fig. 4.

Fig. 4

Exosome-derived miR-122 affects cisplatin-induced AKI and HK2 cells pyroptosis by regulating the expression of ELAVL1. (A) IF staining shows expression of caspase-1 (green); Blue fluorescence represents the nucleus (DAPI). Bar, 100 mm. (B–C) The qPCR analysis of miR-122 and ELAVL1 expression. (D) WB analysis of ELAVL1 expression. (E) The qPCR analysis of caspase-1, NLRP3, and GSDMD expression. (F) WB analysis of caspase-1, NLRP3, and GSDMD expression. (G) H&E staining assays. (H) The distribution of GSDMD in kidney tissues as measured using IHC. (I–M) The blood from AKI mice is checked for BUN, serum creatinine, TNF-α, IL-1β, and IL-18 concentration. (N) The qPCR analysis of miR-122 expression. (O) The WB analysis of ELAVL1, caspase-1, NLRP3, and GSDMD expression. Data are shown as mean ± SEM (n = 3). Asterisks indicate significant differences from the control (*P < 0.05; **P < 0.01; ***P < 0.001).