Table 2. Primer sequences and annealing temperatures used for amplification of mitochondrial DNA in four genes of Echinococcus multilocularis.
| Gene fragment/ ct gene | Primer designation | Primer sequence (5´- 3´) | Annealing temperature | Reference |
|---|---|---|---|---|
| cob (603 bp) | cob-F | TGCTGATTTGTTAAAGTTAGTGATC | 54°C | [22] |
| cob-R | CATAAATCAATGGAAACAACAACAAG | |||
| nad2* (882 bp) | nad2-F | GCGTTGATTCATTGATACATTGT | 55°C | [12] |
| nad2-R | TAGTAAAGCTCAAACCGAGTTCT | |||
| cox1 (789 bp) | F1 | TTGAATTTGCCACGTTTGAATGC | 55°C | [23] |
| R3 | TTGAATTTGCCACGTTTGAATGC | |||
| nad1 (395 bp) | Cest1 | TGCGTTATTGGCATATGGTAG | 60°C | [24] |
| Cest2 | GTGCCACCCTCAGTTGGTACT |
*nad2 –complete (ct) gene analyzed