Table 3. Nucleotide bases in cob, nad2 and cox1 genes detected in Slovak isolates in polymorphic sites determined for European E1 –E5 haplotypes of Echinococcus multilocularis [12].
cob | cob | 603 bp | nad2 | nad2 | 882 bp | cox1 | cox1 | cox1 | cox1 | 789 bp | 2,274 bp | |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Isolate No. | 244 G/T | 369 A/G | Haplotype (cob) | 72 A/G | 486 A/G | Haplotype (nad2) | 22 C/T | 40 T/G | 44 T/C | 203 A/G | Haplotype (cox1) | Final Haplotype |
SK1 | G | G | E5 | G | G | E5 | T | G | C | A | E2, E4, E5 | E5 |
SK2 | G | A | E1, E2, E4 | A | A | E1, E2 | – | – | – | – | – | E1/E2 |
SK3 | G | G | E5 | G | G | E5 | T | G | C | A | E2, E4, E5 | E5 |
SK4 | G | G | E5 | G | G | E5 | T | G | C | G* | E2, E4, E5 | E5a |
SK5 | G | A | E1, E2, E4 | G | A | E3, E4 | T | G | C | A | E2, E4, E5 | E4 |
SK6 | G | G | E5 | G | G | E5 | T | G | C | A | E2, E4, E5 | E5 |
SK7 | G | A | E1, E2, E4 | G | A | E3, E4 | T | G | C | A | E2, E4, E5 | E4 |
SK8 | G | A | E1, E2, E4 | G | A | E3, E4 | T | G | C | A | E2, E4, E5 | E4 |
SK9 | G | G | E5 | G | G | E5 | – | – | – | – | – | E5 |
SK10 | G | G | E5 | G | G | E5 | T | G | C | A | E2, E4, E5 | E5 |
SK11 | G | G | E5 | G | G | E5 | T | G | C | A | E2, E4, E5 | E5 |
SK12 | G | G | E5 | – | – | – | – | – | – | – | – | E5 |
SK13 | G | G | E5 | G | G | E5 | T | G | C | A | E2, E4, E5 | E5 |
SK14 | G | G | E5 | G | G | E5 | T | G | C | G* | E2, E4, E5 | E5a |
SK15 | G | A | E1, E2, E4 | G | A | E3, E4 | T | G | C | A | E2, E4, E5 | E4 |
SK16 | G | G | E5 | G | G | E5 | T | G | C | A | E2, E4, E5 | E5 |
SK17 | – | – | – | G | G | E5 | – | – | – | – | – | E5 |
SK18 | G | G | E5 | G | G | E5 | T | G | C | A | E2, E4, E5 | E5 |
SK19 | G | G | E5 | G | G | E5 | T | G | C | A | E2, E4, E5 | E5 |
SK20 | G | G | E5 | G | G | E5 | T | G | C | A | E2, E4, E5 | E5 |
SK21 | G | G | E5 | G | G | E5 | – | – | – | – | – | E5 |
The dash ("–") with a grey background indicates that no satisfactory amplification was achieved with this gene.
*Additional polymorphism (behind the referenced E1 –E5 polymorphism) in cox1.