TABLE 1.
Response of T cells and B cells to mitogens is suppressed by rLTB but not by rLTB/G33D, a non-GM1-binding mutanta
| Treatment | Mean cpm ± SD (% of control)b for:
|
||
|---|---|---|---|
| CD8+ T cells | CD4+ T cells | Ly5+ B cells | |
| Medium | 34,589 ± 760 | 106,678 ± 4,960 | 40,728 ± 1,160 |
| rLTB (10 μg/ml) | 422 ± 123* (0.2) | 29,994 ± 5,322* (28.1) | 8,249 ± 1,585* (20.3) |
| rLTB/G33D (10μg/ml) | 37,241 ± 330 (108) | 132,215 ± 1,115 (124) | 38,591 ± 1,586 (93.7) |
a
CD8+ (94%), CD4+ (91%), and Ly5+ (96%) cells were positively selected from the spleens of B10.BR mice and activated with immobilized anti-CD3 MAb (T cells) or LPS (B cells) (see Materials and Methods). rLTB and rLTB/G33D were present throughout the 72-h incubation period.
b
Data are for triplicate microwells labeled with [3H]TdR for the last 20 h of culture. *, P < 0.0001 versus medium control and rLTB/G33D.