FIGURE 2.

DEX treatment promoted polarization of astrocytes from the A1 to A2 phenotype in the hippocampus of neonatal after HIBD. (A) Dual immunofluorescence staining was performed to examine the number of A1 astrocytes labeling C3 and GFAP. (B) Quantification for the ratio of C3⁺/GFAP⁺ cells to the total number of GFAP⁺ cells in the dentate gyrus of the hippocampus. (C) Dual immunofluorescence staining was performed to examine the number of A2 astrocytes labeling S100A10 and GFAP. (D) Quantification for the ratio of S100A10⁺/GFAP⁺ cells to the total number of GFAP⁺ cells in the dentate gyrus of the hippocampus. DEX, dexmedetomidine; HI, hypoxic–ischemia; HIBD, hypoxic–ischemic brain damage. Data were expressed as the mean ± SD (n = 5 per group); ^# p < 0.05 vs. the HI group.