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BMMΦs cytokine expression and osteoclastogenic differentiation in response to MC4exo exposure. (A) Cd86, Cd206, Il1β, Il10, Ccl2, Tnfα, Nos and Rankl mRNA expressions of BMMΦ cultured with MC4exo at 20 h and day 7 post exposure. (B) TRAP staining of BMMΦs cultured in the presence of vehicle or RANKL plus vehicle of MC4exo and quantification of the number of TRAP+ osteoclasts (≥3 nuclei).
