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. 2023 Nov 6;28(1):e18029. doi: 10.1111/jcmm.18029

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© 2023 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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MC4exo effects on the osteogenic capacity of calvarial osteoblasts. (A) The effects of MC4exo on the mineralized tissue associated gene expression of calvarial osteoblasts on days 5, 10, 15 and 20: Collagen type I (Col1a1), Bone GLA protein (Bglap), Alkaline phosphatase (Alp), Osteoprotegerin (Opg), Runx2 and Rankl mRNA expressions. (B) Von Kossa staining of calvarial osteoblasts treated with MC4exo or vehicle every 2d for 21d. Image J was used to analyse mineralized nodules. Results are from two independent experiments. n = 6/group.