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. 2023 Nov 6;28(1):e18029. doi: 10.1111/jcmm.18029

FIGURE 5.

FIGURE 5

Intratibial injection of MC4exo had no impact on osteoblast frequency but reduced osteoclasts and macrophages at the experimental end‐point. Histological and immunofluorescent analysis of the tibia. (A) TRAP staining in the diaphysis and quantification of was performed on tibia paraffin sections and analysis included. Representative TRAP+ surface per trabecular bone surface in the metaphysis and diaphysis stained images. Black arrows indicate TRAP+ osteoclasts. Reduced TRAP staining was observed in the diaphysis of the tibia. (B) Immunofluorescent staining for osteocalcin (Ocn) (red) was performed. n = 12 (Vehicle); n = 12 (MC4exo). Scale bar; 500 μm. No significant difference was noted. (C) Immunofluorescent staining for osterix (green) was performed. n = 12 (Vehicle); n = 12 (MC4exo). No significant difference was found. (D) Immunofluorescent staining for F4/80 was performed. Significant reduction was found in the diaphysis of the tibia. ∆: Vehicle, ●: MC4exo. Scale bar: 500 μm.