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. 2023 Nov 1;28(1):e18020. doi: 10.1111/jcmm.18020

FIGURE 6.

FIGURE 6

AKT agonist IGF‐1 reversed KIRREL silencing induced PI3K/AKT/mTOR pathway inhibition and GC cell proliferation and angiogenesis. (A, B) After IGF‐1 (50 ng/mL) was added to lentivirus transfected SNU‐5 cells, the expressions of related proteins in the PI3K/AKT/mTOR pathway, HIF‐1α and VEGF were increased (C) The CCK‐8 results showed that the proliferation ability of GC cells was also enhanced. (D, E) Tube formation assay showed that the ability of angiogenesis of SNU‐5 cells transfected with lentivirus was also significantly enhanced when IGF‐1 was added; scale bar: 100 μm. (F) The viability of HUVECs was examined by CCK8. (G, H) The flow cytometry showed that IGF‐1 had no significant effect on the cycle distribution of SNU‐5 cells transfected with lentivirus (repetition = 3; *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significant difference).