Skip to main content
. 2023 Nov 1;28(1):e18020. doi: 10.1111/jcmm.18020

FIGURE 7.

FIGURE 7

AKT inhibitor LY294002 reversed KIRREL overexpression induced the PI3K/AKT/mTOR pathway activation and GC cell proliferation and angiogenesis. (A, B) After LY294002 (5 μM) was added to AGS cells transfected with lentivirus, the expressions of related proteins in the PI3K/AKT/mTOR pathway, HIF‐1α and VEGF were decreased. (C) The CCK‐8 results showed that the proliferation ability of GC cells was also weakened. (D, E) Tube formation assay showed that the ability of angiogenesis in AGS cells transfected with lentivirus was also significantly reduced after LY294002 was added; scale bar: 100 μm. (F) The viability of HUVECs was examined by CCK8. (G, H) The results of flow cytometry showed that the S‐cycle of AGS cells transfected with lentivirus was shortened, while the G0/G1 and G2/M cycles were prolonged after LY294002 was added (repetition = 3; *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significant difference).