Development of pontine DMG model
(A) Up panel, Bolt coordinates in relation to lambda and sagittal sutures. Down panel, photo of mouse skull with bolt positioned in DIPG coordinates.
(B) Up panel, schematic drawing of screw guide components used in this administration method. Down panel, a macroscopic photograph of ink solution injected at 6.5 mm of depth with a Hamilton syringe through the bolt. Adapted from Marigil et al.2