Figure 4.

Implantation of the guide-screw system

(A) Prepare the surgery by sterilizing the head of the mouse with betadine.

(B) Prepare the mouse for the implantation surgery by making an incision into the shaved and clean scalp using a fresh scalpel blade.

(C) Make a small mark according to the coordinates mentioned (1.0 mm right to lambda and 0.8 mm posterior) with a small 15-gauge needle.

(D) Drill a hole over the mark with a hand-held twist drill the skull.

(E) Introduce the bolt/screw with its specific screwdriver by applying slight pressure throughout the previous twist hole.

(F) Representative image of how it should fit implanted in the skull.