Dissociation medium

Reagent	Final concentration	Amount
X GI	N/A	500 mL
Collagenase IV	1 mg/mL	500 mg
DNase I	40 μg/mL	20 mg
Total	N/A	500 mL

Store at −20°C for 6 months, pre-warm in a water bath at 37°C before use.