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. 2023 Dec 29;5(1):102803. doi: 10.1016/j.xpro.2023.102803

FACS staining intracellular antibody

Antibody Clone Fluorochrome Dilution factor Amount/100 μL staining mix
FOXP3 FJK-16S PE-eFluor 610 1:100 1 μL
PermWash 1× N/A N/A N/A 99 μL
Total N/A N/A N/A 100 μL

Prepare the solution immediately before use, protect it from light, and keep it on ice.