REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
AF750 anti-mouse CD45 (30-F11), dilution (1:200)	BioLegend	Cat#103128; RRID: AB_493715
APC anti-mouse F4/80 (BM8), dilution (1:200)	BioLegend	Cat#123116; RRID: AB_893481
BUV395 anti-mouse CD11b (M1/70), dilution (1:200)	BD Biosciences	Cat#563553; RRID: AB_2738276
BUV496 anti-mouse CD4 (GK1.5), dilution (1:200)	BioLegend	Cat#612952; RRID: AB_2813886
BV421 anti-mouse CD19 (6D5), dilution (1:400)	BioLegend	Cat#115538; RRID: AB_11203527
BV510 anti-mouse CD8a (53-6.7), dilution (1:100)	BioLegend	Cat#107520
BV605 anti-mouse NK1.1 (PK136), dilution (1:200)	BioLegend	Cat#141721; RRID: AB_2562273
BV650 anti-mouse IA/IE (M5/114.15.2), dilution (1:300)	BioLegend	Cat#108739; RRID: AB_2565975
BV785 anti-mouse TCRb (H57-597), dilution (1:100)	BioLegend	Cat#107641; RRID: AB_2810347
FITC anti-mouse Ly6C (1A8), dilution (1:100)	BioLegend	Cat#151211; RRID: AB_1186135
PECy7 anti-mouse CD11c (N418), dilution (1:200)	BioLegend	Cat#119703; RRID: AB_493568
PE-eFluor 610 anti-mouse FOXP3 (FJK-16S), dilution (1:200)	Thermo Fisher Scientific	Cat#124227; RRID: AB_2574624
PerCP/Cy5.5 anti-mouse Ly6G (PK136), dilution (1:200)	BioLegend	Cat#505821; RRID: AB_1877271
PromoFluor-840 maleimide, dilution (1:10,000)	AAT Bioquest	Cat#1402
Chemicals, peptides, and recombinant proteins
Cell culture water	Sigma-Aldrich	Cat#W3500-500ML
DPBS (with calcium, magnesium, glucose, pyruvate)	Thermo Fisher Scientific	Cat#14287080
Fetal bovine serum (FBS)	Gibco	Cat#10270106
RPMI medium	Gibco	Cat#61870-010
Penicillin-Streptomycin (10.000 U/mL)	Thermo Fisher Scientific	Cat#15140122
EDTA, 0.5 M sterile solution, pH 8.0	VWR	Cat#E177-100ML
FcR Block	BD Biosciences	Cat#553141
Percoll stock solution	Sigma-Aldrich	Cat#GE17-0891-01
Scalpel	Salunatur	#Cat0210
Ophthalmic “lipolac”	Bausch + Lomb	#Cat764118.6
Ketamidor (ketamine)	Richter Pharma	N/A
Rompun (xylazine)	Bayer	N/A
Buprenorphine	Acofarma	#Cat961425
Antisedan	Pfizer	#CatPF61
Hystoacryl	B. Braun	#Cat10520010
Critical commercial assays
Foxp3/transcription factor staining buffer	eBioscience	Cat#00-5523-00
Experimental models: Cell lines
Murine DIPG: NP53	Laboratory of Dr. Oren Becher	N/A
Murine DIPG: XFM	Laboratory of Dr. Oren Becher	N/A
Experimental models: Organisms/strains
Mouse: NP53 fl/fl (NTV-a p53floxed); age: 4 weeks; gender: male and female (1:1)	Laboratory of Dr. Oren Becher	N/A
Mouse: BALB/cJ; strain #:000651; age: 4 weeks; gender: male and female (1:1)	The Jackson Laboratory	Strain #:000651
Software and algorithms
FlowJo 10.8.1	BD Biosciences	N/A
Prism software 9.3.1	GraphPad by Domatics	N/A
Other
Bolts (guide-screw system)	Plastics One	#CatC212SG
Hamilton syringe	Thermo Fisher Scientific	#Cat87900
PHD 2000 infusion syringe pump multiple syringe holder	Harvard Apparatus	#CatHPHD2000IWMSRR
1 mm diameter drill bit	Plastics One drill HSS	#Cat8J60
Screwdriver	Plastics One	N/A
96-well clear V-bottom sterile microplate	Corning	Cat#P-96-450V-C
Cell strainer, pore size 70 mm	Sarstedt	Cat#83.3945.070
5 mL round bottom polystyrene tubes (FACS tubes)	Corning	Cat#352054
50 mL Falcon tubes	Sarstedt	Cat#62547004
15 mL Falcon tubes	Sarstedt	Cat#430791
gentleMACS C tubes	Miltenyi	Cat#130-093-237
gentleMACS Octo dissociator with heaters	Miltenyi	Cat#130-096-427
MACSmix tube rotator	Miltenyi	Cat#130-090-753
CytoFLEX LX	Beckman Coulter	N/A