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. 2024 Jan 23;15:680. doi: 10.1038/s41467-023-44178-y

Fig. 3. Tissue DEL-1 expression is not suppressed upon sepsis in neonates and controls neonatal tissue neutrophil infiltration.

Fig. 3

a DEL-1 relative mRNA expression in lung tissue from C57BL/6 mice of adult (8–10 weeks) or neonatal age (4 days old) upon polymicrobial sepsis [6 and 12 hours after cecal slurry (CS) administration] (expression of DEL-1 in adult control lungs was set as 1) (n = 9 animals in adult 0 h, n = 6 animals in adult 6 h, n = 7 animals in adult 12 h, n = 10 animals in neonates 0 h, n = 7 animals in neonates 6 h and n = 5 animals in neonates 12 h group). b DEL-1 protein levels in lung lysates from C57BL/6 mice of adult (8–10 weeks) or neonatal age (4 days old) following 12 hours of CS-induced polymicrobial sepsis (n = 7 animals in adult control, n = 5 animals in adult sepsis and neonate control groups and n = 6 animals in neonatal sepsis group). c DEL-1 relative mRNA expression in small intestine from C57BL/6 mice of adult (8–10 weeks) or neonatal age (4 days old) following 6 hours of CS-induced polymicrobial sepsis (expression of DEL-1 in adult control intestines was set as 1) (n = 4 animals in adult control group, n = 6 animals in adult sepsis group, n = 5 animals in neonate control group and n = 6 animals in neonatal sepsis group). d Myeloperoxidase (MPO) activity in total lung lysates in wild type (WT) C57BL/6 adult and neonate mice 6 and 12 hours after CS-induced sepsis (n = 6 animals in adult 0 h, n = 6 animals in adult 6 h, n = 4 animals in adult 12 h, n = 6 animals in neonates 0 h, n = 7 animals in neonates 6 h and n = 5 animals in neonates 12 h group). e Neutrophil  numbers (CD11b+ Ly6G+) in the peritoneum in WT C57BL/6 adult and neonate mice 6 and 12 hours after CS-induced sepsis (n = 6 animals in adult 0 h, n = 6 animals in adult 6 h, n = 5 animals in adult 12 h, n = 8 animals in neonates 0 h, n = 6 animals in neonates 6 h and n = 5 animals in neonates 12 h group). f MPO activity in lung (n = 8 animals in WT +IgG-Fc control, n = 5 animals in Del1−/− + IgG-Fc control, n = 5 animals in Del1−/− + DEL-1-Fc control, n = 7 animals in WT + IgG-Fc sepsis, n = 6 animals in Del1−/− + IgG-Fc sepsis and n = 5 animals in Del1−/− + DEL-1-Fc sepsis group). and g total neutrophil count (CD11b+ Ly6G+) in peritoneal lavage in WT and Del1−/− C57BL/6 neonate mice that received either DEL-1-Fc or IgG-Fc i.v. 15 min prior to CS-induced sepsis (6 hours) (n = 8 animals in WT +IgG-Fc control, n = 6 animals in Del1−/− + IgG-Fc control, n = 5 animals in Del1−/− + DEL-1-Fc control and n = 8 animals per group in WT + IgG-Fc sepsis, Del1−/− + IgG-Fc sepsis and Del1−/− + DEL-1-Fc sepsis groups). Mean ± SD (a–d) is depicted. Statistical analysis by one-way ANOVA with Bonferroni’s multiple comparison post-test (a–g), and two-sided unpaired t test (a and c, between adult mice indicated with #). Source data are provided as a Source Data file. Ctl control, h hours, U units, g grams.