Fig. 6. DEL-1 supports bone marrow neutrophil pool in neonatal sepsis.

a DEL-1 protein expression (pg/ml) per 10⁶ bone marrow cells from wild type (WT) C57BL/6 mice of adult and neonatal age at basal state and following 12 hours of cecal slurry (CS) -induced sepsis (n = 5 animals per group in adult control, neonate control, and adult sepsis group and n = 4 animals in neonate sepsis group). b DEL-1 mRNA relative expression in total bone marrow cells from WT C57BL/6 mice of adult and neonatal age at basal state and following 6 and 12 hours of CS-induced sepsis (expression of DEL-1 in adult control mice was set as 1) (n = 24 animals in adult control, n = 20 animals in neonate control, n = 7 animals in adult 6 h sepsis, n = 6 animals in neonate 6 h sepsis, n = 6 animals in adult 12 h sepsis and n = 9 animals in neonate 12 h sepsis group). c Total neutrophil numbers (CD11b⁺Ly6G⁺Ly6C⁻, normalized per femur and per mouse weight in grams) in bone marrow of WT adult, WT and Del1^−/− C57BL/6 neonate pups in steady state and following 6 and 12 hours of CS induced sepsis (n = 7 animals in WT adult control group, n = 5 animals per group in WT adult 6 h and 12 h sepsis, n = 16 animals in WT neonate control, n = 10 animals per group in WT neonate 6 h and 12 h sepsis, n = 7 animals in Del1^−/− control, n = 9 animals in Del1^−/− 6 h sepsis and n = 10 animals in Del1^−/− 12 h sepsis). d Total neutrophil numbers (CD11b⁺Ly6G⁺Ly6C⁻, normalized per femur and per mouse weight in grams) in bone marrow of WT neonates, and Del1^−/− neonate pups treated with i.v. DEL-1-Fc or IgG-Fc 15 min prior to injection of CS and following 12 hours of CS-induced sepsis (n = 5 animals per group). Mean ± SD is depicted (a–d). Statistical analysis by one-way ANOVA with Bonferroni’s and multiple comparison post-test (a, b, d) and two-sided unpaired t test between the indicated groups (c). Source data are provided as a Source Data file. Ctl control, Ad adults, Neo neonate, h hours, g grams.