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. 2024 Jan 23;150(1):27. doi: 10.1007/s00432-023-05511-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Elevated DNA damage in cervical epithelium. A Indirect immunofluorescent staining for Ki67, γH2AX in cervical epithelium. Nuclei were counterstained by DAPI. Stages of CIN and cervical cancer and magnifications (200 × and 1000x) are indicated. IF: inflammatory lesion. B, C As in (A), immunostaining for phosphorylated ATM (pS1981, B) and 53BP1 (C) in nuclear compartment of cervical epithelial cells. pS1981 displayed cytoplasmic staining in inflammatory lesions and CIN I, which we regarded as non-specific. All cervical samples were diagnosed as HPV16-positive according to pathological examination (see methods)