Fig. 2.

The HPV-E7 oncogene interferes with DDR. A Expression of HA-tagged E6 and E7 in HEK293T cells. pLV, empty vector. B Immunoblotting for ATM and ATR-dependent checkpoint phosphor-signals in 293T expressing E7 or not. Cells were challenged with IR (10 Gy) for indicated time. C–E Immunoblotting showing indicated phosphorylation of NBS1 and RPA32, or H2B monoubiquitination upon expression of E6 (C) and E7 (C, D). 293 T cells were insulted with CPT or IR (4 Gy) for indicated time. E7 expression was determined by α-HA immunoblotting