Fig. 2. Identification of transcriptionally-defined cell types in mouse LS.

A Schematic of experimental design for snRNA-seq of mouse LS tissue. Tissues from 2 mice of the same sex were pooled together for each individual sample for a total of N = 4 samples (generated from n = 4 male, 4 female mice). B Uniform manifold approximation and projection (UMAP) of identified cell types, with nuclei counts per clusters. Feature plots for expression of (C) Snap25, (D) Gad2, (E) Slc1a2, and (F) Mbp across the dataset. G Heatmap of cell type markers used to characterize the dataset with transformed, normalized expression values (logcounts), centered and scaled.