FIGURE 2.

Development of GLUT9^rs16890979, GLUT9^OE, and GLUT9^shRNA H9 cell lines. (A) Schematic diagram showing the wildtype and genetically manipulated H9 cell lines used in this study. (B) Global frequencies and distribution of the SLC2A9 rs16890979 polymorphism according to the National Library of Medicine database. (C) Schematic diagram for the CRISPR/Cas9 homologous gene repair template we used. (D) Sequencing of wild-type and gene-edited SLC2A9. (E) Schematic diagram of the lentivirus plasmid to overexpress SLC2A9. (F) Schematic diagram of the shRNA lentivirus plasmid for targeting SLC2A9. (G) SLC2A9 mRNA expression in each H9 cell line assessed by qRT-PCR. (H, I) SLC2A9 protein expression in each H9 cell line assessed by Western blotting.