. 2024 Jan 23;108(1):167. doi: 10.1007/s00253-024-13005-9

Table 2.

FAs composition (%) of initial substrates (GD Gainesville diet, T tangerine, S strawberry, O orange, FDM fresh dairy manure)

Fatty acids	GD	S	T	O	FDM	SEM	p value
C4:0	0	0	0	0	7.09	-	-
C12:0	0.28^a	0.12^c	0.78^a	0.40^a	0.37^a	0.17	< 0.0001
C14:0	0.50^d	0.37^d	2.37^b	0.76^c	2.73^a	0.80	< 0.0001
C14:1	0	0.03^b	0	0	2.85^a	1.42	0.0471
C15:0	0	0.13^b	0.61^ab	0	1.45^a	0.50	0.0110
C16:0	15.47^c	8.43^d	19.23^b	22.85^a	19.51^b	3.93	< 0.0001
C16:1	0.61^d	0.67^d	1.97^a	2.05^a	1.57^c	0.50	< 0.0001
C17:0	0	0.11^b	0.12^b	0.83^a	0.89^a	0.31	0.0004
C18:0	9.52^b	1.76^d	4.92^c	2.91^d	16.31^a	4.24	< 0.0001
C18:1 CIS9	14.92^c	22.37^a	19.76^b	13.06^d	18.1^b	2.65	< 0.0001
C18:2 CIS n6 (LA)	42.09^a	35.05^b	15.03^d	32.28^b	19.26^c	8.03	< 0.0001
C18:3 n3 (ALA)	14.19^c	28.86^a	30.93^a	23.93^b	0.6^d	8.89	< 0.0001
C18:3 n6	0	0	0	0	5.20	-	-
C20:0	0.46^b	0.87^b	0.79^b	0	3.68^a	1.25	< 0.0001
C20:1	0.92^a	0.29^a	0	0	0	0.40	0.2053
C20:3 n6	0	0	0	0	2.33	-	-
C20:4 n6 (AA)	0.01^b	0.07^b	0.06^b	0	0.54^a	0.18	< 0.0001
C20:5 n3 (EPA)	0	0.04^a	0	0	0.18^a	0.07	0.1290
C22:0	0.28^a	0.43^a	2.27^b	0	2.94^b	0.97	0.0002
C22:1	0.25^a	0	0	0	0.04^b	0.11	0.0235
C22:6 n3 (DHA)	0.004^a	0	0	0	1.14^a	0.44	0.1163
C24:0	0.28^c	0.15^c	1.12^a	0.93^ab	0.62^b	0.30	0.0001
	GD	S	T	O	FDM	SEM
SFAs	25.79^c	12.46^d	32.21^b	28.68^c	56.09^a	11.30	< 0.0001
MUFAs	16.71^bc	23.43^a	21.77^ab	15.11^c	24.47^a	3.03	0.0016
PUFAs	56.50^b	64.20^a	46.02^c	56.21^b	28.96^d	9.69	< 0.0001
n − 6	42.11^a	35.30^b	15.09^e	32.28^c	27.23^d	7.22	< 0.0001
n − 3	14.39^b	28.90^a	30.93^a	23.93^b	1.73^c	8.57	< 0.0001

FA composition was evaluated by gas chromatography. Data are presented as mean of three biological replicates ± SEM and analyzed by one-way ANOVA and Tuckeypost-hoc test. For C14:1, C20:1, C20:5 n3 (EPA), C22:1 and C22:6, statistical analysis was performed with unpaired t-test with Welch’s correction. Different letters indicate significant differences among groups (P < 0.01)