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. 2024 Jan 10;11:1308879. doi: 10.3389/fcell.2023.1308879

FIGURE 3.

FIGURE 3

VVL staining of frozen and FFPE sections (A) Representative images of bladder cancers. VVL (brown) and nuclei (blue) are shown. Areas circled by red dotted lines indicate blood vessels. (a) Low-grade non-invasive urothelial carcinoma; (b) Low- and high-grade non-invasive urothelial carcinoma; (c) High-grade invasive urothelial carcinoma; (d) High-grade invasive urothelial carcinoma; (e) Muscle invasive urothelial carcinoma, sarcomatoid variant; (f) Muscle invasive urothelial carcinoma, plasmacytoid variant; (g) Small-cell carcinoma. Scale bars = 50 μm. (B) Scoring of VVL staining intensity was performed using the ImageJ software. Data represent the mean ± SEM of each three spot of each photo.