Table 2.
Residues of Sho-Stl protein that differ from the corresponding Sa-Stl residues within the Sa-Stl: human dUTPase complex interface based on crystal structure (PDB ID: 7PWJ).
| hDUT | Sa-Stl | Sho-Stl | ||
|---|---|---|---|---|
| Residue-atom | Residue-atom | Type of interaction with hDUT | Corresponding residue | Potential interaction with hDUT |
| I119-Cγ2 | E59-Cδ | vdW | N53 | – |
| F135-Cβ | I67-Cγ2 | vdW | L61 | Possible |
| F135-Cε | P68-Cδ | vdW | S62 | Possible |
| 28Y-OH | R74-NH1 | H-bond | K68 | Possible |
|
133G-C and 134G-Cα |
S99- Cβ | vdW | N91 | Potential steric clash |
| D127-Oδ1 | N107-Oδ1 | H-bond | K99* | Potential steric clash |
| G131-O | N107-Nδ2 | H-bond | K99* | – |
| G87-O | Y113-OH | H-bond | F105* | – |
| G87-N | Y113-OH | H-bond | F105* | – |
*Residue mutated in Sho-Stl-NDY construct.