TABLE 1.
Bacterial strains and plasmids used in this study
| Strain, phage, or plasmid | Relevant properties | Source or reference |
|---|---|---|
| P. aeruginosa strains | ||
| PA103 | Wild-type cytotoxic respiratory clinical isolate | 1, 27 |
| Noncytotoxic mutants of PA103 | Summarized in Table 2 | 22 |
| E. coli strains | ||
| S17-1 | thi pro hsdR recA RP4-2(Tet::Mu) (Km::Tn7) | 47 |
| XL1-Blue MRF′ | Δ(mcrA)183 Δ(mcrCB hsdSMR mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proAB lacIqZΔM15 Tn10(Tetr)] | Stratagene Corp. |
| Plasmids and cosmids | ||
| pBR322 | Cloning vector | Gibco BRL |
| pBluescriptII | Cloning vector | Stratagene Corp. |
| pLAFR5SK1 | Cosmid vector; pLAFR5 (23) with improved polylinker site | L. Shapiro |
| 4-7-H | pLAFR5SK1 cosmid with PA103 genomic DNA insert | This study |
| 4-11-H | pLAFR5SK1 cosmid with PA103 genomic DNA insert | This study |
| 5-9-E | pLAFR5SK1 cosmid with PA103 genomic DNA insert | This study |
| pAH830 | pBluescriptII containing the 2.0-kb SmaI-SmaI fragment of cosmid 4-7-H | This study |
| pAH831 | pBluescriptII containing the 8.0-kb EcoRI-EcoRI fragment of cosmid 4-7-H | This study |