FIG. 2.
PCR products generated with primers designed to be complementary to the sequence of stn flanking the predicted site of insertion of Kmr (STN1 and STN4) and with template DNA from wild-type Salmonella strains (lanes 2, 4, 6, and 8) or from stn mutants (lanes 3, 5, 7, and 9). Lane 1, standard markers; lanes 2 and 3, S. typhimurium ST4/74; lanes 4 and 5, S. typhimurium ST12/75; lanes 6 and 7, S. dublin SD2229; lanes 8 and 9, S. dublin SD3246; lane 10, negative control. Sizes of standard markers are shown on the left.