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. 2024 Jan 24;43:31. doi: 10.1186/s13046-024-02956-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — CDH17 and DSC1 regulate p120-catenin isoform 1 expression. A, B The indicated cell lines were transfected with control, DSC1 or CDH17 targeting siRNAs. Cell extracts were analyzed by Western blot to detect the indicated proteins. The indicated protein expression levels were significantly increased (*, p < 0.05; **, p < 0.01) or decreased (◊◊, p < 0.01; ◊◊◊, p < 0.001) after siRNA transfection. C The same cell lines were transfected with empty vectors (Mock) or vectors encoding for CDH17 RAD, and the cell extracts analyzed as before. D, E The indicated transfectants were subjected to subcellular fractionation and analyzed by Western blot. RhoGDI, EGFR and Lamin B were used as loading controls for cytosolic, membrane and nuclear fractions, respectively. The expression levels of p120-catenin isoform 1 in each subcellular fraction were significantly increased (*, p < 0.05; **, p < 0.01) or decreased (◊, p < 0.05) after DSC1 silencing or CDH17 RAD overexpression. Data are representative of three independent experiments