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BCG Tokyo culture fluid was separated by SDS-PAGE in an 8 to 18% gradient gel. Each lane contained 10 μg of total protein. +, reducing conditions with ME in the application buffer; −, no ME. Numbers at the left indicate molecular masses in kilodaltons. Staining is indicated above the lanes. CBB, BCG Tokyo culture fluid stained with Coomassie brilliant blue. PAbs, polyvalent anti-BCG antibodies (included as a control) and anti-MPB70 (K34); MAbs, 1-5C, 1-1D, Bov-1, and MBS43.
