TABLE 1.
Effect of signaling inhibitors on cytotoxicity induced by P. aeruginosa 6206
| Treatment | No. of affected cells (trypan blue stained)
|
|
|---|---|---|
| Controla | Treated (P) | |
| Genistein (200 μM)b | 468 ± 44 | 79 ± 2 (0.0001) |
| Genistein (200 μM)c (cell pretreatment only) | 241 ± 27 | 174 ± 15 (0.04) |
| Tyrphostin 47 (50 μM) | 359 ± 122 | 72 ± 23 (0.004) |
| Herbimycin A (5 μM)d | 339 ± 66 | 314 ± 50 (0.38) |
| Wortmannin (50 nM) | 187 ± 70 | 179 ± 55 (0.86) |
a
Cells were not treated with drug.
b
Cells were pretreated with genistein for 1 h, and then the drug was also added during incubation with bacteria.
c
Cells were only pretreated with genistein (for 1 h).
d
Cells were pretreated with herbimycin A for 3.5 h, and then the drug was also added during incubation with bacteria.