Extended Data Fig. 10 |. Mutagenesis of murine caspase-11 based on structural and sequence alignments.
a, Sequence alignment of human caspase-4 and murine caspase-11. Identical and similar amino acids are highlighted in red or white boxes, respectively. Sequences were aligned using ClustalOmega and plotted in ESPript 3.0. b, c, d, Immunoblots showing in vitro cleavage of human pro-IL-18 by caspase-11 mutants. Immunoblots are representative of three biological replicates. For gel source data, see Supplementary Figure 1.