Extended Data Table 1.
Data Collection, data processing and validation statistics
| Data Collection and Processing | |
|---|---|
|
| |
| Microscope | Titan Krios |
| Voltage (keV) | 300 |
| Camera | K3 |
| Magnification | 105,000 |
| Pixel size at detector (Å/pixel) | 0.83 |
| Total electron exposure (e−/Å2) | 60.8 |
| Exposure rate (e−/pixel/sec) | 28.6 |
| Number of frames collected during exposure | 50 |
| Defocus range (μm) | −1.0 to −2.5 |
| Automation software | SerialEM 3.8 |
| Energy filter slit width (eV) | 20 |
| Micrographs collected (no.) | 5,934 |
| Micrographs used (no.) | 28,309 |
| Total extracted particles (no.) | 10,802,676 |
| Refinement | |
| Refined particles (no.) / Final particles (no.) | 1,234,038/229,147 |
| Symmetry parameters | C2 |
| Map resolution (Å) | 3.2 |
| FSC 0.143 (unmasked / masked) | 4.0/3.2 |
| Resolution range (Å) | 2.9 to 11.1 |
| Resolution range due to anisotropy (Å) | 3.3 to 3.7 |
| Map sharpening B factor range (Å2) | −144.2 |
| Map sharpening methods | LocalDeblur |
| Model composition | |
| Chains | 6 |
| Protein residues | 816 |
| Validation | |
| Model-Map scores | |
| CC (correlation coefficients) | 0.77 |
| Average FSC (0 / 0.143 / 0.5) | 2.2/3.3/3.5 |
| R.m.s. deviations from ideal values | |
| Bond lengths (Å) | 0.004 |
| Bond angles (°) | 0.787 |
| MolProbity score | 1.85 |
| CaBLAM outliers | 3.02 |
| Clashscore | 6.81 |
| Poor rotamers (%) | 0.26 |
| C-beta outliers (%) | 0.00 |
| Ramachandran plot | |
| Favoured (%) | 92.36 |
| Allowed (%) | 7.64 |
| Outliers (%) | 0.00 |