Fig 3.

Effect of PvCE15 on glucose release during a 1-h saccharification of certain pectin-rich biomasses. Biomass materials of (A) carrot pomace, (B) potato peels, and (C) orange peels were milled, washed, and resuspended in assays of 5 mg/mL and incubated for 1 h at 30°C with either UltraFlo (UF), PvCE15 (CE15), pectate lyase (PL), or pectin methyl esterase (PME) and were compared to control reactions (C) lacking the addition of enzymes. Released monosaccharides from assays (N = 4) were quantified by ion chromatography, and the differences in means were evaluated by Student’s t-test. The addition of PvCE15 to either UltraFlo or UltraFlo supplemented with the pectate lyase did not lead to significant increases (ns) in released glucose, while the addition of PME to the reactions with UltraFlo supplemented with the pectate lyase did for both carrot pomace and potato peels but not orange peels. The washed orange peel control samples contained a considerable amount of free glucose that was not present before the 1-h incubation, indicating additional factors were contributing to monosaccharide release during the assays.