TABLE 1.

Summary of kinetics of the studied CE15 enzymes with model substrates

Enzyme	Substrate	Km (mM)	kcat (s−1)	kcat/Km (/s−1/mM−1)
AsCE15	BnzGlcA	2.63 ± 0.45	31.57 ± 1.63	11.99 ± 2.12
	MeGlcA	2.91 ± 0.48	19.85 ± 0.87	6.82 ± 1.17
	AllylGlcA	3.06 ± 0.43	21.17 ± 0.79	6.91 ± 1.00
	MeGalA	9.03 ± 2.08	1.35 ± 0.12	0.15 ± 0.04
	BnzGalA	6.26 ± 0.42	1.92 ± 0.04	0.31 ± 0.02
PdCE15	BnzGlcA	0.91 ± 0.12	120.07 ± 3.98	131.79 ± 17.8
	MeGlcA	1.27 ± 0.12	122.32 ± 3.25	96.59 ± 9.61
	AllylGlcA	1.05 ± 0.11	133.54 ± 3.65	127.47 ± 13.77
	MeGalA	4.83 ± 0.25	39.33 ± 0.76	8.14 ± 0.45
	BnzGalA	1.18 ± 0.09	72.38 ± 1.75	61.49 ± 4.86
BiCE15	BnzGlcA	1.68 ± 0.27	77.04 ± 3.38	45.88 ± 7.59
	MeGlcA	2.26 ± 0.20	57.65 ± 1.69	25.55 ± 2.37
	AllylGlcA	2.37 ± 0.29	63.92 ± 2.76	26.95 ± 3.5
	MeGalA	Could not be saturated		0.14 ± 0.011
	BnzGalA	Could not be saturated		0.35 ± 0.028
PvCE15	BnzGlcA	0.73 ± 0.09	96.66 ± 3.42	131.63 ± 16.33
	MeGlcA	1.44 ± 0.16	78.83 ± 2.43	54.62 ± 6.10
	AllylGlcA	0.97 ± 0.06	86.38 ± 1.75	89.18 ± 5.91
	MeGalA	5.70 ± 0.29	36.33 ± 0.93	6.37 ± 0.37
	BnzGalA	1.07 ± 0.09	49.01 ± 1.20	45.66 ± 4.01
PiCE15A	BnzGlcA	0.57 ± 0.11	6.73 ± 0.29	11.86 ± 2.38
	MeGlcA	3.83 ± 0.64	4.99 ± 0.35	1.3 ± 0.23
	AllylGlcA	1.71 ± 0.17	6.48 ± 0.19	3.8 ± 0.38
	MeGalA	2.49 ± 0.49	2.59 ± 0.14	1.04 ± 0.21
	BnzGalA	0.54 ± 0.05	5.11 ± 0.10	9.49 ± 0.87