Table 1.
Selected lectins (n = 9) for capturing the common glycan epitopes of therapeutic IgG mAbs.
| # | Lectin (origin) | Reported epitope selectivity relevant to the benchmark N-glycan epitope* | Targeting N-glycan epitope on IgG mAb |
|---|---|---|---|
| 1 | Recombinant PhoSL or rPhoSL (Pholiota squarrosa) | α(1,6)fucosylated N-glycans18 | Core fucose (Fuc) |
| 2 | rOTH3 (Ulva limnetica) | Unknown (see Supplemental Table S2)19 | Terminal N-acetylglucosamine (GlcNAc) |
| 3 | RCA120 (Ricinus communis) | Galβ(1,4)GlcNAc (up with increasing the number of terminal β-Gal), Galβ(1,3)Gal (weak), no affinity for agalactosylated N-type and α-galactose | Terminal β-galactose (β-Gal) |
| 4 | rMan2 (Kappaphycus alvarezii) | High Mannose (High Man)13 | High mannose (High Man) |
| 5 | MAL_I (Maackia amurensis) | Siaα(2,3)Galβ(1,4)GlcNAc | Terminal α2,3-linked sialic acids; primarily N-acetylneuraminic acid (NANA) in CHO-produced mAbs |
| 6 | rPSL1a | Siaα(2,6)Galβ(1,4)GlcNAc20 | Terminal α2,6-linked sialic acids, primarily N-glycolylneuraminic acid (NGNA) in murine cell produced mAbs |
| 7 | PHAE (Phaseolus vulgaris) | Bi-antennary complex-type N-glycan with outer β-Gal and bisecting GlcNAc (up with increasing the number of terminal β-galactose), no affinity for fully sialylated N-type | Bisecting GlcNAc |
| 8 | rMOA (Marasmius oreades) | α-Gal (Galα(1,3)Galβ(1,4)GlcNAc),21 no affinity for β-galactose | α-galactose (α-Gal) |
| 9 | PHAL (Phaseolus vulgaris) | Tri/tetra-antennary complex-type N-glycan | Triantennary N-glycan |
*For naturally occurring lectins, refer to Lectin Frontier DataBase (LfDB)(https://acgg.asia/lfdb2/).