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Southern blot analysis of genomic DNA extracted from GAS A-20 and SW507. The probes used were specific for the speB gene and λ DNA. The DNAs were digested with NsiI (lanes 1 and 4), HindIII (lanes 2 and 5), and HindIII-NsiI (lanes 3 and 6). Lanes 1 to 3, GAS A-20 genomic DNA; lanes 4 to 6, GAS SW507 genomic DNA; lanes M, λ HindIII marker used as a molecular size standard.
