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Western immunoblot analysis of SPE B present in the supernatants of wild-type GAS (A-20 and NZ131) and mutants (SW507 and SW510) after electrophoresis on an 8% polyacrylamide gel. The blot was developed with rabbit anti-SPE B polyclonal antibodies. Lane 1, A-20; lane 2, SW507; lane 3, NZ131; lane 4, SW510; lane 5, purified 28-kDa SPE B from A-20 used as a standard. The mature SPE B protein is indicated (arrow).
