Skip to main content
. 2022 Dec 12;17(2):195–201. doi: 10.4103/ajts.ajts_33_21

Figure 1.

Figure 1

Flowcytometric enumeration of CD34+ cells using international society of hematotherapy and graft engineering single platform: (a) Debris are gated in FSC-A versus SSC-A plot (all events are considered). (b) Non-debris population is selected and beads are gated. (c) Then non-beads events are plotted with through probe PerCp cy 5.5 in FSC versus SSC-A, the viable cells are then gated. (d and e) Out of those viable cells CD45+ cells are taken and lymphocytes are gated to check the position of blasts cells i.e., dim for CD45+ but behind the lymphocytes. (f) Out of these viable CD45+ cells plot, another graph is made with CD34PE versus SSC-A and bright CD34+ cells are gated and named as P1. (g) The population of cells positive for CD45 cells is named as P2 cells. (h) These P1 cells, dim for CD45 (as seen in e) are then represented on FSC-A versus SSC-A plot and bright cluster is gated and includes viable CD34+ cells. * FSC = Forward Scatter, SSC = Side Scatter, (*Correlation is significant at 0.01 level (2-tailed))