Figure 7. Canonical pathways altered in Dmp1^KO osteoblasts are differentially regulated by FGF23 and by DMP1.

Bulk RNA-sequencing analysis was performed on bone marrow stromal cells isolated from 12-week-old WT (n = 3), Fgf23^Dmp1-cKO (n = 3), Dmp1^KO (n = 3), and Dmp1^KO Fgf23^Dmp1-cKO (n = 3) mice and cultured for 21 days in osteoblast differentiation medium containing 10 mM of beta-glycerophosphate. (A) Venn diagram identifies genes showing altered expression in Dmp1^KO but not in Dmp1^KO Fgf23^cKO osteoblasts (tan area). Heatmaps represent the expression of (B) Fgf23 and (C) genes identified in A and used in Ingenuity Pathway Analysis (IPA; QIAGEN) to define the most represented canonical pathways regulated by FGF23. (D) Venn diagram identifies genes showing altered expression in Dmp1^KO and in Dmp1^KO Fgf23^cKO osteoblasts (burgundy area). Heatmaps represent the expression of (E) Dmp1 and (F) genes identified in D and used in IPA to define the most represented canonical pathways regulated by DMP1. Statistical tests were unpaired t test and corrected by the false discovery rate (P < 0.1).