Figure 8. Osteocyte-specific deletion of Fgf23 restores osteoblast differentiation in Dmp1^KO at the single-cell level.

Single-cell RNA-sequencing analysis was performed on bone marrow stromal cells isolated from 12-week-old WT (n = 3), Fgf23^Dmp1-cKO (n = 3), Dmp1^KO (n = 3), and Dmp1^KO Fgf23^Dmp1-cKO (n = 3) mice and cultured for 21 days in osteoblast differentiation medium containing 10 mM of beta-glycerophosphate. Uniform manifold approximation and projection (UMAP) plot of (A) total isolated cells and (B) osteoblast lineage cells only. (C) Dot plot of cluster-enriched markers in osteoblastic cells segregated by clusters of differentiation. Pseudotime analysis of osteoblastic cluster from (D) all groups combined and (E) segregated by group. (F) Percentage of osteoblast-like cells in the total number of isolated cells segregated by cluster of differentiation and by group.