Fig. 5. Comparison of ML-designed libraries D2 and D3 to the NNK library.

(A) Experimental titers (viral genome/ml) plotted against the MPLE. ***P < 0.001 compared to NNK. (B) Comparison of the effective number of variants present in each library after packaging and post-packaged libraries are labeled with the “-post” suffix. (C) Experimental titers and effective number of variants for D2, D3, and NNK DNA libraries (pre-packaging selection), and the NNK-post library (post-packaging selection). The NNK-post library represents the NNK library after one round of packaging selection. One-way ANOVA followed by Tukey test (**P < 0.01 compared to D2). In all cases, experimental titers are measured on three biological replicates. Graphs show means ± SD.