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. 2024 Jan 24;10(4):eadj3880. doi: 10.1126/sciadv.adj3880

Fig. 2. Expression and roles of TSP-1 in membrane barrier integrity in adults and during aging.

Fig. 2.

(A to D) Representative epifluorescence images showing TSP-1::GFP up-regulation from L4 (A) to young adults (B) and during aging (C) and (D). (E) Quantification of fluorescence intensities of TSP-1::GFP under conditions indicated. ***P < 0.001 (n > 30 animals per condition). (F) Schematic of the assay for acute membrane barrier functions. (G) Quantification of intensities of fluorescein accumulated by live animals under conditions indicated. ***P < 0.001 (n > 20 animals per condition). (H to J) Representative epifluorescence images showing fluorescein accumulation in animals of indicated stages, temperature and genotypes (wild-type and tsp-1 deletion mutant allele ok3594). Scale bars, 100 μm.