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. 2024 Jan 24;10(4):eadj3880. doi: 10.1126/sciadv.adj3880

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Fig. 4. — (A) Representative confocal images showing colocalization of the marker Akt-PH::GFP, which binds to intestinal apical plasma membrane inner leaflet PIP₂/PIP₃, with endogenous TSP-1 tagged with wrmScarlet by CRISPR. Scale bars, 10 μm. (B) Fluorescent intensity correlation analysis showing close proximity of Akt-PH::GFP and TSP-1::wrmScarlet. (C) Representative confocal images showing non-colocalization of the marker ERm::GFP, which labels intestinal ER membrane, with endogenous TSP-1 tagged with wrmScarlet by CRISPR. Scale bars, 10 μm. (D) Fluorescent intensity correlation analysis for ERm::GFP and TSP-1::wrmScarlet. (E) Representative confocal time-series images showing stability of tetraspanin webs formed by endogenous TSP-1::wrmScarlet, with enlarged views in (F). Scale bars, 10 μm.