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. 2024 Jan 24;10(4):eadj3880. doi: 10.1126/sciadv.adj3880

Fig. 6. Gain of function of TSP-1 confers longevity extension in C. elegans and thermal resilience in human cells.

Fig. 6.

(A) Schematic of the transgene that produces tsp-1 gain-of-function OE lines (OE line 1 at low level, OE line 2 at high level as measured by qRT-PCR). ***P < 0.001. (B) Life-span curves of wild type and two representative tsp-1 OE lines at 28°C starting at L4. A total of 50% median and 16% maximal survival extension by overexpression of tsp-1 in wild type, ***P < 0.001 (n > 40 animals per condition). (C) Life-span curves of wild-type and tsp-1 OE lines at constant 20°C. A total of 63% median and 24% maximal life-span extension by overexpression of tsp-1 in wild type, ***P < 0.001 (n > 40 animals per condition). (D) Serial confocal fluorescence images showing tetraspanin web-like structures formed in the human cell line HEK293 by CMV promoter-driven expression of C. elegans tsp-1::GFP (bottom) but not GFP alone (top). Scale bar, 10 μm. (E) Quantification of survival rates after heat shock in HEK293 cells showing enhanced thermal resilience conferred by ectopic gain-of-function of C. elegans TSP-1::GFP. ***P < 0.001 (three independent biological replicates).