Appendix 1—figure 2. The distribution of pyrimethamine-P. falciparum dihydrofolate reductase (PfDHFR) binding free energy changes predicted by Flex ddG for 250 runs for subset of mutations considered in Sirawaraporn et al., 1997, namely (a) N51I, (b) C59R, (c) S108N, (d) I164L, (e) N51I,S108N, (f) C59R,S108N, (g) N51I,C59R,S108N, (h) C59R,S108N,I164L, and (i) N51I,C59R,S108N,I164L.