TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| L. pneumophila serogroup 1 (Philadelphia) | ATCC 33152; type strain | CDCa |
| L. longbeachae serogroup 1 | ATCC 33462; type strain | CDC |
| L. longbeachae serogroup 2 | ATCC 33484; type strain | CDC |
| A5H5 (L. longbeachae serogroup 1) | Australian clinical isolate | This study |
| B10 | ATCC 33462 L. longbeachae serogroup 1 strain with a mip deletion mutation | This study |
| B8 | L. longbeachae serogroup 1 A5H5 with a mip deletion mutation | This study |
| B8.22 | Strain B8 complemented with plasmid SKW27 | This study |
| E. coli DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rk− mk+) deoR thi-1 supE44 λ− gyrA96 relA1 | 20 |
| E. coli S17-1 | recA derivative of E. coli 294 (hsdR Pro) with RP4-2Tc::Mu (Ap Km Nm Tc::Mu) Km::Tn7 in the chromosome | 39 |
| Plasmids | ||
| pGEM-7Zf(−) | Apr cloning vector | Promega |
| pUC18K | pUC18 with the aphA-3 Kmr resistance cassette | P. Sansonetti, reference 31 |
| pCACTUS | Cmr cloning vector containing sacB and a temperature-sensitive replicon | C. A. Clark |
| pIMVS26 | pGEM with ca. 8-kb fragment of L. longbeachae serogroup 1 ATCC 33462 genomic DNA | This study |
| pIMVS27 | pGEM carrying a SacI fragment of pIMVS26 | This study |
| pIMVS28 | pGEM containing deleted mip gene generated in pIMVS27 | This study |
| pCACTUS49 | pCACTUS containing deleted mip gene fragment from pIMVS28 | This study |
| pCACTUS50 | Derivative of pCACTUS49 containing Kmr from pUC18K and mob deletion | This study |
| pWKS130 | Kmr cloning, sequencing vector | 46 |
| pIMVS29 | pWKS130 containing entire mip gene on SacI fragment from pIMVS27 | This study |
a
CDC, Centers for Disease Control and Prevention, Atlanta, Ga.