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. 2023 Nov 20;123(1):68–79. doi: 10.1016/j.bpj.2023.11.019

Figure 4.

Figure 4

NanoDSF measurements for DsbB reconstituted in dark nanodiscs under reducing conditions. (A) The F350/F330 thermal unfolding curve of DsbB reconstituted in the dark nanodisc in the presence of the reducing agent TCEP at 2 mM. (B) The first derivative plot shows a single inflection point corresponding to a Tm value of 76.6°C ± 0.5°C. The first derivative plot for DsbB reconstituted in the dark nanodisc in the absence of reducing conditions has been overlayed as a comparison. All samples were run in triplicate and the average is plotted.